Mark Twain Tonight! is a one-man play devised by Hal Holbrook, in which he depicted Mark Twain giving a dramatic recitation selected from several of Twain's writings, with an emphasis on the comic ones.

Harold Rowe Holbrook Jr. was an American actor. He first received critical acclaim in 1954 for a one-man stage show that he developed called Mark Twain Tonight! while studying at Denison University. He won the Tony Award for Best Actor in a Play in 1966 for his portrayal of Twain. He continued to perform his signature role for over 60 years, only retiring the show in 2017 due to his failing health. Throughout his career, he also won five Primetime Emmy Awards for his work on television and was nominated for an Academy Award for his work in film.