The Marquette Interchange is a major freeway interchange in downtown Milwaukee, Wisconsin, located near Marquette University where Interstate 43, I-94, and I-794 meet. Originally built in the 1960s, the interchange underwent a total rebuild between 2004 and 2008, and was officially opened on August 19, 2008, after what was at the time the largest construction project in state history. During the early 2000s, U.S. Highway 41 (US 41) had followed I-94 through the interchange. This had changed when I-41 was designated in the Milwaukee area. US 41 now follows I-41 through the Zoo Interchange instead.

Milwaukee is the most populous city in the U.S. state of Wisconsin and the county seat of Milwaukee County. With a population of 577,222 at the 2020 census, Milwaukee is the 31st-most populous city in the United States, and the fifth-most populous city in the Midwest. It is the central city of the Milwaukee metropolitan area, the 40th-most populous metro area in the U.S. Milwaukee is categorized as a "Gamma minus" city by the Globalization and World Cities Research Network, with a regional GDP of over $102 billion in 2020.