Mathura Lok Sabha constituency is one of the 80 Lok Sabha (parliamentary) constituencies in the Indian state of Uttar Pradesh. This constituency covers the entire Mathura district. According to Election Commission of India 2009 reports, the Mathura Parliamentary constituency has a total of 1,341,649 electorates out of which 593,726 are females and 747,923 are males.

Mathura district situated along the banks of the river Yamuna is a district of Uttar Pradesh state of north-central India. The historic city of Mathura is the district headquarters. Mathura district is home to many important sites associated with goddess Radha and Lord Krishna, who was born in Mathura and grew up in the nearby town of Vrindavan. Both cities are some of the most sacred sites in the Vaishnava tradition, making Mathura district an important Hindu pilgrimage centre.