The Mayflower Compact, originally titled Agreement Between the Settlers of New Plymouth, was the first governing document of Plymouth Colony. It was written by the men aboard the Mayflower, consisting of Separatist Puritans, adventurers, and tradesmen. Although the agreement contained a pledge of loyalty to the King, the Puritans and other Protestant Separatists were dissatisfied with the state of the Church of England, the limited extent of the English Reformation and reluctance of King James I of England to enforce further reform.

Plymouth Colony was the first permanent English colony in New England from 1620 and the third permanent English colony in America, after Newfoundland and the Jamestown Colony. It was settled by the passengers on the Mayflower at a location that had previously been surveyed and named by Captain John Smith. The settlement served as the capital of the colony and developed as the town of Plymouth, Massachusetts. At its height, Plymouth Colony occupied most of the southeastern portion of Massachusetts. Many of the people and events surrounding Plymouth Colony have become part of American folklore, including the American tradition of Thanksgiving and the monument of Plymouth Rock.