Mayflies are aquatic insects belonging to the order Ephemeroptera. This order is part of an ancient group of insects termed the Palaeoptera, which also contains dragonflies and damselflies. Over 3,000 species of mayfly are known worldwide, grouped into over 400 genera in 42 families.

The Holy Family with the Dragonfly, also known as The Holy Family with the Mayfly, The Holy Family with the Locust, and The Holy Family with the Butterfly is an engraving by the German artist Albrecht Dürer (1471–1528) from approximately 1495. It is quite small but full of intricate detail. A very popular image, copied by other printmakers within five years of creation, it is found in most major print room collections, including the Indianapolis Museum of Art and the UK Royal Collection.