Mayo, Florida is a town and the only municipality in Lafayette County, Florida, United States. It is the county seat of Lafayette County. The population was 1,055 at the 2020 census.

Lafayette County is a county located in the north central portion of the state of Florida. As of the 2020 census, the population was 8,226, making it the second-least populous county in Florida. The county seat is Mayo. Lafayette County is a prohibition or partially dry county, allowing retail sales of beer.