The Mayor of Gainesville is, for ceremonially purposes, receipt of service of legal processes and the purposes of military law, official head of the city of Gainesville, Florida and otherwise a member of, and chair of, the city commission, required to preside at all meetings thereof. The mayor is also allowed to vote on all matters that come before the city commission, but has no veto powers.

Gainesville is the county seat of Alachua County, Florida, and the most populous city in North Central Florida, with a population of 145,214 in 2022. It is the principal city of the Gainesville metropolitan area, which had a population of 339,247 in 2020.