Medford is a hamlet and census-designated place (CDP) in the Town of Brookhaven in Suffolk County, on Long Island, in New York, United States. The population was 24,247 at the 2020 census.

Medford is a station in the hamlet of Medford, New York on the Main Line of the Long Island Rail Road. Medford is located on New York State Route 112 between Peconic Avenue and Long Island Avenue. Access to the station is available from a narrow curving roadway leading off Route 112. This roadway used to connected with the Ohio Avenue intersection until Ohio Avenue was closed north of Peconic Avenue in 2007. It is also accessible from the north end of Oregon Avenue, although the Medford Fire Department periodically closes the Oregon Avenue access road for drills or other exercises.