Melbourne University tram stop is a major terminating point and stop of the Melbourne tram system. It features a single island platform and three shunts. It is located on Swanston Street, Carlton, and is the major tram stop for the University of Melbourne, its namesake. It opened in 2005, and is one of the busiest stops on the network; six tram lines terminate at it, while two run through.

Melbourne tram route 64 is operated by Yarra Trams on the Melbourne tram network from Melbourne University to Brighton East. The 18.1 kilometre route is operated out of Glenhuntly depot with Z and B class trams.