Melbourne tram route 109 is operated by Yarra Trams on the Melbourne tram network from Box Hill to Port Melbourne. The 19.3 kilometre route is operated out of Kew depot with A and C class trams.

Yarra Trams is the trading name of the operator of the tram network in Melbourne, Australia, which is owned by VicTrack and leased to Yarra Trams by the Victorian Department of Transport and Planning. The current franchise is operated by Keolis Downer. As at May 2014, Yarra Trams operate 487 trams, across 26 tram routes and a free City Circle tourist tram, over 1,763 tram stops. With 250 km (155.3 mi) of double track, Melbourne's tram network is the largest in the world.