Melbourne tram route 16 is operated by Yarra Trams on the Melbourne tram network from Melbourne University to Kew. The 20.2 kilometre route is operated out of Malvern depot with Z and D1 class trams.

Melbourne University tram stop is a major terminating point and stop of the Melbourne tram system. It features a single island platform and three shunts. It is located on Swanston Street, Carlton, and is the major tram stop for the University of Melbourne, its namesake. It opened in 2005, and is one of the busiest stops on the network; six tram lines terminate at it, while two run through.