Melbourne tram route 16 is operated by Yarra Trams on the Melbourne tram network from Melbourne University to Kew. The 20.2 kilometre route is operated out of Malvern depot with Z and D1 class trams.

Domain Interchange was a major interchange on the Melbourne tram system. It featured two island platforms with four tracks, and had dedicated turning tracks and through tracks. It was located on St Kilda Road between Domain Road and Park Street, adjacent to Kings Domain, and was one of the busiest interchanges on the system, being used by eight tram routes. A shelter built in 1986 was replaced when the stop was rebuilt in 2013.