Mennonites in Paraguay are either Plautdietsch-speakers of mostly Flemish, Frisian and Prussian ancestry or, like the majority of Paraguayans, of mixed or Amerindian ancestry. Ethnic Mennonites contribute heavily to the agricultural and dairy output of Paraguay.

The Russian Mennonites are a group of Mennonites who are the descendants of Dutch and North German Anabaptists who settled in the Vistula delta in West Prussia for about 250 years and established colonies in the Russian Empire beginning in 1789. Since the late 19th century, many of them have emigrated to countries which are located throughout the Western Hemisphere. The rest of them were forcibly relocated, so very few of their descendants currently live in the locations of the original colonies. Russian Mennonites are traditionally multilingual but Plautdietsch is their first language as well as their lingua franca. In 2014, there were several hundred thousand Russian Mennonites: about 200,000 live in Germany, 74,122 live in Mexico, 150,000 in Bolivia, 40,000 live in Paraguay, 10,000 live in Belize, tens of thousands of them live in Canada and the US, and a few thousand live in Argentina, Uruguay, and Brazil.