Messier 100 is a grand design intermediate spiral galaxy in the southern part of the mildly northern Coma Berenices. It is one of the brightest and largest galaxies in the Virgo Cluster and is approximately 55 million light-years from our galaxy, its diameter being 107,000 light years, and being about 60% as large. It was discovered by Pierre Méchain in 1781 and 29 days later seen again and entered by Charles Messier in his catalogue "of nebulae and star clusters". It was one of the first spiral galaxies to be discovered, and was listed as one of fourteen spiral nebulae by Lord William Parsons of Rosse in 1850. NGC 4323 and NGC 4328 are satellite galaxies of M100; the former is connected with it by a bridge of luminous matter.

Coma Berenices is an ancient asterism in the northern sky, which has been defined as one of the 88 modern constellations. It is in the direction of the fourth galactic quadrant, between Leo and Boötes, and it is visible in both hemispheres. Its name means "Berenice's Hair" in Latin and refers to Queen Berenice II of Egypt, who sacrificed her long hair as a votive offering. It was introduced to Western astronomy during the third century BC by Conon of Samos and was further corroborated as a constellation by Gerardus Mercator and Tycho Brahe. It is the only modern constellation named for a historic person.