1.
Microorganism
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A microorganism or microbe is a microscopic organism, which may be single-celled or multicellular. The study of microorganisms is called microbiology, a subject that began with the discovery of microorganisms in 1674 by Antonie van Leeuwenhoek, microorganisms are very diverse and include all bacteria, archaea and most protozoa. This group also contains some fungi, algae, and some such as rotifers. Many macroscopic animals and plants have microscopic juvenile stages, some microbiologists classify viruses and viroids as microorganisms, but others consider these as nonliving. In July 2016, scientists identified a set of 355 genes from the last universal ancestor of all life, including microorganisms. Microorganisms, under certain test conditions, have observed to thrive in the vacuum of outer space. Microorganisms likely far outweigh all other living things combined, the mass of prokaryote microorganisms including the bacteria and archaea may be as much as 0.8 trillion tons of carbon, out of the total biomass of between 1 and 4 trillion tons. Microorganisms appear to thrive in the Mariana Trench, the deepest spot in the Earths oceans, in August 2014, scientists confirmed the existence of microorganisms living 800 m below the ice of Antarctica. According to one researcher, You can find microbes everywhere — theyre extremely adaptable to conditions, microorganisms are crucial to nutrient recycling in ecosystems as they act as decomposers. As some microorganisms can fix nitrogen, they are a part of the nitrogen cycle. Microorganisms are also exploited in biotechnology, both in food and beverage preparation, and in modern technologies based on genetic engineering. A small proportion of microorganisms are pathogenic, causing disease and even death in plants, Robert Hooke coined the term cell after viewing plant cells under his microscope. Antonie Van Leeuwenhoek was one of the first people to observe microorganisms in 1673, later, in the 19th century, Louis Pasteur found that microorganisms caused food spoilage, debunking the theory of spontaneous generation. In 1876 Robert Koch discovered that microorganisms cause diseases, single-celled microorganisms were the first forms of life to develop on Earth, approximately 3–4 billion years ago. Further evolution was slow, and for about 3 billion years in the Precambrian eon, so, for most of the history of life on Earth, the only forms of life were microorganisms. Bacteria, algae and fungi have been identified in amber that is 220 million years old, microorganisms tend to have a relatively fast rate of evolution. Most microorganisms can reproduce rapidly, and bacteria are able to freely exchange genes through conjugation, transformation and transduction. This rapid evolution is important in medicine, as it has led to the development of multidrug resistant pathogenic bacteria, superbugs, the possible existence of microorganisms was discussed for many centuries before their discovery in the 17th century
2.
Growth medium
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A growth medium or culture medium is a solid or liquid or semi-solid designed to support the growth of microorganisms or cells, or small plants like the moss Physcomitrella patens. Different types of media are used for growing different types of cells, the most common growth media for microorganisms are nutrient broths and agar plates, specialized media are sometimes required for microorganism and cell culture growth. Some organisms, termed fastidious organisms, require specialized environments due to nutritional requirements. Viruses, for example, are intracellular parasites and require a growth medium containing living cells. The most common media for microorganisms are nutrient broths or LB medium. Liquid media are often mixed with agar and poured via a media dispenser into Petri dishes to solidify. These agar plates provide a medium on which microbes may be cultured. They remain solid, as very few bacteria are able to decompose agar, bacteria grown in liquid cultures often form colloidal suspensions. In the case of cells, this difficulty is often addressed by the addition of blood serum or a synthetic serum replacement to the medium. In the case of microorganisms, no such limitations exist, as they are often unicellular organisms. One other major difference is that cells in culture are often grown on a flat surface to which they attach, and the medium is provided in a liquid form. In contrast, bacteria such as Escherichia coli may be grown on solid or in liquid media, an important distinction between growth media types is that of defined versus undefined media. A defined medium will have known quantities of all ingredients, for microorganisms, they consist of providing trace elements and vitamins required by the microbe and especially defined carbon and nitrogen sources. Glucose or glycerol are used as carbon sources, and ammonium salts or nitrates as inorganic nitrogen sources. An undefined medium has some complex ingredients, such as yeast extract or casein hydrolysate, undefined media are sometimes chosen based on price and sometimes by necessity – some microorganisms have never been cultured on defined media. A good example of a medium is the wort used to make beer. The wort contains all the nutrients required for yeast growth, and under anaerobic conditions, when the fermentation process is complete, the combination of medium and dormant microbes, now beer, is ready for consumption. Minimal media can also be used to select for or against recombinants or exconjugants and this supplementation allows for the culturing of specific lines of auxotrophic recombinants
3.
Cell (biology)
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The cell is the basic structural, functional, and biological unit of all known living organisms. A cell is the smallest unit of life that can replicate independently, the study of cells is called cell biology. Cells consist of cytoplasm enclosed within a membrane, which contains many such as proteins. Organisms can be classified as unicellular or multicellular, while the number of cells in plants and animals varies from species to species, humans contain more than 10 trillion cells. Most plant and animal cells are only under a microscope. The cell was discovered by Robert Hooke in 1665, who named the unit for its resemblance to cells inhabited by Christian monks in a monastery. Cells emerged on Earth at least 3.5 billion years ago, Cells are of two types, eukaryotic, which contain a nucleus, and prokaryotic, which do not. Prokaryotes are single-celled organisms, while eukaryotes can be either single-celled or multicellular, prokaryotic cells were the first form of life on Earth, characterised by having vital biological processes including cell signaling and being self-sustaining. They are simpler and smaller than eukaryotic cells, and lack membrane-bound organelles such as the nucleus, prokaryotes include two of the domains of life, bacteria and archaea. The DNA of a prokaryotic cell consists of a chromosome that is in direct contact with the cytoplasm. The nuclear region in the cytoplasm is called the nucleoid, most prokaryotes are the smallest of all organisms ranging from 0.5 to 2.0 µm in diameter. Though most prokaryotes have both a cell membrane and a wall, there are exceptions such as Mycoplasma and Thermoplasma which only possess the cell membrane layer. The envelope gives rigidity to the cell and separates the interior of the cell from its environment, the cell wall consists of peptidoglycan in bacteria, and acts as an additional barrier against exterior forces. It also prevents the cell from expanding and bursting from osmotic pressure due to a hypotonic environment, some eukaryotic cells also have a cell wall. Inside the cell is the region that contains the genome, ribosomes. The genetic material is found in the cytoplasm. Prokaryotes can carry extrachromosomal DNA elements called plasmids, which are usually circular, linear bacterial plasmids have been identified in several species of spirochete bacteria, including members of the genus Borrelia notably Borrelia burgdorferi, which causes Lyme disease. Though not forming a nucleus, the DNA is condensed in a nucleoid, plasmids encode additional genes, such as antibiotic resistance genes
4.
Species
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In biology, a species is the basic unit of biological classification and a taxonomic rank. A species is defined as the largest group of organisms in which two individuals can produce fertile offspring, typically by sexual reproduction. While this definition is often adequate, looked at more closely it is problematic, for example, with hybridisation, in a species complex of hundreds of similar microspecies, or in a ring species, the boundaries between closely related species become unclear. Other ways of defining species include similarity of DNA, morphology, all species are given a two-part name, a binomial. The first part of a binomial is the genus to which the species belongs, the second part is called the specific name or the specific epithet. For example, Boa constrictor is one of four species of the Boa genus, Species were seen from the time of Aristotle until the 18th century as fixed kinds that could be arranged in a hierarchy, the great chain of being. In the 19th century, biologists grasped that species could evolve given sufficient time, Charles Darwins 1859 book The Origin of Species explained how species could arise by natural selection. Genes can sometimes be exchanged between species by horizontal transfer, and species may become extinct for a variety of reasons. In his biology, Aristotle used the term γένος to mean a kind, such as a bird or fish, a kind was distinguished by its attributes, for instance, a bird has feathers, a beak, wings, a hard-shelled egg, and warm blood. A form was distinguished by being shared by all its members, Aristotle believed all kinds and forms to be distinct and unchanging. His approach remained influential until the Renaissance, when observers in the Early Modern period began to develop systems of organization for living things, they placed each kind of animal or plant into a context. Many of these early delineation schemes would now be considered whimsical, animals likewise that differ specifically preserve their distinct species permanently, one species never springs from the seed of another nor vice versa. In the 18th century, the Swedish scientist Carl Linnaeus classified organisms according to shared physical characteristics and he established the idea of a taxonomic hierarchy of classification based upon observable characteristics and intended to reflect natural relationships. At the time, however, it was widely believed that there was no organic connection between species, no matter how similar they appeared. However, whether or not it was supposed to be fixed, by the 19th century, naturalists understood that species could change form over time, and that the history of the planet provided enough time for major changes. Jean-Baptiste Lamarck, in his 1809 Zoological Philosophy, described the transmutation of species, proposing that a species could change over time, in 1859, Charles Darwin and Alfred Russel Wallace provided a compelling account of evolution and the formation of new species. Darwin argued that it was populations that evolved, not individuals and this required a new definition of species. Darwin concluded that species are what appear to be, ideas
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Seaweed
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Seaweed refers to several species of macroscopic, multicellular, marine algae. The term includes some types of red, brown, and green algae, seaweed can also be classified by use. Seaweed is a term and lacks a formal definition. A seaweed may belong to one of groups of multicellular algae, the red algae, green algae. As these three groups do not have a common ancestor, the seaweed are in a polyphyletic group. In addition, some tuft-forming bluegreen algae are sometimes considered to be seaweed, seaweeds appearance somewhat resembles non-arboreal terrestrial plants. Two specific environmental requirements dominate seaweed ecology and these are the presence of seawater and the presence of light sufficient to drive photosynthesis. Another common requirement is an attachment point, although some genera such as Sargassum. As a result, seaweed most commonly inhabit the part of a sea that is close to the shore, seaweed occupy a wide range of ecological niches. The highest elevation is only wetted by the tops of sea spray, in some areas, littoral seaweed can extend several miles out to sea. The limiting factor in such cases is sunlight availability, the deepest living seaweed are some species of red algae. Others have adapted to live in rock pools. In this habitat seaweed must withstand rapidly changing temperature and salinity, seaweed has a variety of purposes, for which it is farmed or foraged from the wild. At the beginning of 2011, Indonesia produced 3 million tonnes of seaweed and surpassed the Philippines as the worlds largest seaweed producer, by 2011, the production was estimated to have reached 10 million tonnes. Seaweed is consumed by people, particularly in East Asia, e. g. Japan, China, Korea, Taiwan. In Asia, Gim, nori, zicai are sheets of dried Porphyra used in soups, chondrus crispus is another red alga used in producing food additives, along with Kappaphycus and gigartinoid seaweed. Porphyra is a red alga used in Wales to make laver, laverbread, made from oats and the laver, is a popular dish there. In northern Belize, edible seaweed are mixed with milk, nutmeg, cinnamon, seaweed are also harvested or cultivated for the extraction of alginate, agar and carrageenan, gelatinous substances collectively known as hydrocolloids or phycocolloids
6.
Cyanobacteria
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The name cyanobacteria comes from the color of the bacteria. Sometimes, they are called blue-green algae, and incorrectly so, because cyanobacteria are prokaryotes, like other prokaryotes, cyanobacteria have no membrane-sheathed organelles. Photosynthesis is performed in distinctive folds in the membrane of the cell. Biologists commonly agree that chloroplasts found in eukaryotes have their ancestry in cyanobacteria, via a process called endosymbiosis, Cyanobacteria are a group of photosynthetic, nitrogen fixing bacteria that live in a wide variety of habitats such as moist soils and in water. They may be free-living or form relationships with plants or with lichen-forming fungi as in the lichen genus Peltigera. They range from unicellular to filamentous and include colonial species, colonies may form filaments, sheets, or even hollow balls. Cyanobacteria can fix nitrogen in anaerobic conditions by means of specialized cells called heterocysts. Heterocysts may also form under the environmental conditions when fixed nitrogen is scarce. Free-living cyanobacteria are present in the column in rice paddies, and cyanobacteria can be found growing as epiphytes on the surfaces of the green alga, Chara. Cyanobacteria such as, can provide rice plantations with biofertilizer, many cyanobacteria form motile filaments of cells, called hormogonia, that travel away from the main biomass to bud and form new colonies elsewhere. The cells in a hormogonium are often thinner than in the state. To break away from the parent colony, a hormogonium often must tear apart a weaker cell in a filament, each individual cell of a cyanobacterium typically has a thick, gelatinous cell wall. They lack flagella, but hormogonia of some species can move about by gliding along surfaces, many of the multicellular filamentous forms of Oscillatoria are capable of a waving motion, the filament oscillates back and forth. In water columns, some cyanobacteria float by forming gas vesicles and these vesicles are not organelles as such. They are not bounded by membranes, but by a protein sheath. Cyanobacteria can be found in almost every terrestrial and aquatic habitat—oceans, fresh water, damp soil, temporarily moistened rocks in deserts, bare rock and soil and they can occur as planktonic cells or form phototrophic biofilms. They are found in almost every endolithic ecosystem, a few are endosymbionts in lichens, plants, various protists, or sponges and provide energy for the host. Some live in the fur of sloths, providing a form of camouflage, aquatic cyanobacteria are known for their extensive and highly visible blooms that can form in both freshwater and marine environments
7.
Virginia
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Virginia is a state located in the Mid-Atlantic region of the United States, as well as in the historic Southeast. The geography and climate of the Commonwealth are shaped by the Blue Ridge Mountains and the Chesapeake Bay, the capital of the Commonwealth is Richmond, Virginia Beach is the most populous city, and Fairfax County is the most populous political subdivision. The Commonwealths estimated population as of 2014 is over 8.3 million, the areas history begins with several indigenous groups, including the Powhatan. In 1607 the London Company established the Colony of Virginia as the first permanent New World English colony, slave labor and the land acquired from displaced Native American tribes each played a significant role in the colonys early politics and plantation economy. Although the Commonwealth was under one-party rule for nearly a century following Reconstruction, the Virginia General Assembly is the oldest continuous law-making body in the New World. The state government was ranked most effective by the Pew Center on the States in both 2005 and 2008 and it is unique in how it treats cities and counties equally, manages local roads, and prohibits its governors from serving consecutive terms. Virginias economy changed from agricultural to industrial during the 1960s and 1970s. Virginia has an area of 42,774.2 square miles, including 3,180.13 square miles of water. Virginias boundary with Maryland and Washington, D. C. extends to the mark of the south shore of the Potomac River. The southern border is defined as the 36° 30′ parallel north, the border with Tennessee was not settled until 1893, when their dispute was brought to the U. S. Supreme Court. The Chesapeake Bay separates the portion of the Commonwealth from the two-county peninsula of Virginias Eastern Shore. The bay was formed from the river valleys of the Susquehanna River. Many of Virginias rivers flow into the Chesapeake Bay, including the Potomac, Rappahannock, York, and James, the Tidewater is a coastal plain between the Atlantic coast and the fall line. It includes the Eastern Shore and major estuaries of Chesapeake Bay, the Piedmont is a series of sedimentary and igneous rock-based foothills east of the mountains which were formed in the Mesozoic era. The region, known for its clay soil, includes the Southwest Mountains around Charlottesville. The Blue Ridge Mountains are a province of the Appalachian Mountains with the highest points in the state. The Ridge and Valley region is west of the mountains and includes the Great Appalachian Valley, the region is carbonate rock based and includes Massanutten Mountain. The Cumberland Plateau and the Cumberland Mountains are in the southwest corner of Virginia, in this region, rivers flow northwest, with a dendritic drainage system, into the Ohio River basin
8.
Belgium
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Belgium, officially the Kingdom of Belgium, is a sovereign state in Western Europe bordered by France, the Netherlands, Germany, Luxembourg, and the North Sea. It is a small, densely populated country which covers an area of 30,528 square kilometres and has a population of about 11 million people. Additionally, there is a group of German-speakers who live in the East Cantons located around the High Fens area. Historically, Belgium, the Netherlands and Luxembourg were known as the Low Countries, the region was called Belgica in Latin, after the Roman province of Gallia Belgica. From the end of the Middle Ages until the 17th century, today, Belgium is a federal constitutional monarchy with a parliamentary system of governance. It is divided into three regions and three communities, that exist next to each other and its two largest regions are the Dutch-speaking region of Flanders in the north and the French-speaking southern region of Wallonia. The Brussels-Capital Region is a bilingual enclave within the Flemish Region. A German-speaking Community exists in eastern Wallonia, Belgiums linguistic diversity and related political conflicts are reflected in its political history and complex system of governance, made up of six different governments. Upon its independence, declared in 1830, Belgium participated in the Industrial Revolution and, during the course of the 20th century, possessed a number of colonies in Africa. This continuing antagonism has led to several far-reaching reforms, resulting in a transition from a unitary to a federal arrangement during the period from 1970 to 1993. Belgium is also a member of the Eurozone, NATO, OECD and WTO. Its capital, Brussels, hosts several of the EUs official seats as well as the headquarters of major international organizations such as NATO. Belgium is also a part of the Schengen Area, Belgium is a developed country, with an advanced high-income economy and is categorized as very high in the Human Development Index. A gradual immigration by Germanic Frankish tribes during the 5th century brought the area under the rule of the Merovingian kings, a gradual shift of power during the 8th century led the kingdom of the Franks to evolve into the Carolingian Empire. Many of these fiefdoms were united in the Burgundian Netherlands of the 14th and 15th centuries, the Eighty Years War divided the Low Countries into the northern United Provinces and the Southern Netherlands. The latter were ruled successively by the Spanish and the Austrian Habsburgs and this was the theatre of most Franco-Spanish and Franco-Austrian wars during the 17th and 18th centuries. The reunification of the Low Countries as the United Kingdom of the Netherlands occurred at the dissolution of the First French Empire in 1815, although the franchise was initially restricted, universal suffrage for men was introduced after the general strike of 1893 and for women in 1949. The main political parties of the 19th century were the Catholic Party, French was originally the single official language adopted by the nobility and the bourgeoisie
9.
Pasteur Institute
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The Pasteur Institute is a French non-profit private foundation dedicated to the study of biology, micro-organisms, diseases, and vaccines. It is named after Louis Pasteur, who some of the greatest breakthroughs in modern medicine at the time, including pasteurization and vaccines for anthrax. The institute was founded on June 4,1887, and inaugurated on November 14,1888, for over a century, the Institut Pasteur has been at the forefront of the battle against infectious disease. This worldwide biomedical research organization based in Paris was the first to isolate HIV, the Institut Pasteur was founded in 1887 by Louis Pasteur, the famous French chemist and microbiologist. He was committed both to basic research and its practical applications, as soon as his institute was created, Pasteur brought together scientists with various specialties. One year after the inauguration of the Institut Pasteur, Roux set up the first course of microbiology ever taught in the world, the early exploitation of his discovery might have saved millions of lives, especially in World War I. The discovery and use of sulfonamides in treating infections was another breakthrough, some researchers won fame by discovering antitoxins and Daniel Bovet received the 1957 Nobel Prize for his discoveries on synthetic anti-histamines and curarizing compounds. Since World War II, Pasteur researchers have focused on molecular biology. Their achievements were recognized in 1965, when the Nobel Prize was shared by François Jacob, Jacques Monod, in 1985, the first human vaccine obtained by genetic engineering from animal cells, the vaccine against hepatitis B, was developed by Pierre Tiollais and collaborators. During the First World War the Institute was not only involved in the prevention of sanitary risks but also had to deal with the demands of the moment. The most urgent matter was to vaccinate the troops against typhoid fever, easily contracted by the soldiers who often had no choice, by September 1914, the Institute was able to provide 670,000 doses of the needed vaccine and continued to produce it throughout the conflict. Thats how Michel Weinberg, Metchnikoff’s scholar, disclosed the complex etiology of gas gangrene, the First World War involved science in warfare, a movement of active participation arose among researchers who felt the need to help France win the war. In 1938 the Institute, despite its poverty, built a biochemical division and another one dedicated to cellular pathology. During the same period, Andre Lwoff assumed the direction of a new microbial physiology branch built on rue Dutot. This relative freedom allowed the Institute to become, during the two years after the occupation, a pharmacy for the Resistance thanks to the initiative of Vallery-Radot. The Germans became suspicious of the Institute’s staff only after an outbreak of typhoid in a Wehrmacht division that was stationed near Paris before being sent to the Russian front. The fact that the epidemic spread after the Germans sold some of the butter to civilians was proof that the breakout was not caused by local water quality. Wolmann and his wife, as well as other three lab assistants, to a concentration camp, the causes of the decadence that brought the Institute to financial ruins were numerous, but most of them were associated with its commercial and industrial activities and its management
10.
Paris
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Paris is the capital and most populous city of France. It has an area of 105 square kilometres and a population of 2,229,621 in 2013 within its administrative limits, the agglomeration has grown well beyond the citys administrative limits. By the 17th century, Paris was one of Europes major centres of finance, commerce, fashion, science, and the arts, and it retains that position still today. The aire urbaine de Paris, a measure of area, spans most of the Île-de-France region and has a population of 12,405,426. It is therefore the second largest metropolitan area in the European Union after London, the Metropole of Grand Paris was created in 2016, combining the commune and its nearest suburbs into a single area for economic and environmental co-operation. Grand Paris covers 814 square kilometres and has a population of 7 million persons, the Paris Region had a GDP of €624 billion in 2012, accounting for 30.0 percent of the GDP of France and ranking it as one of the wealthiest regions in Europe. The city is also a rail, highway, and air-transport hub served by two international airports, Paris-Charles de Gaulle and Paris-Orly. Opened in 1900, the subway system, the Paris Métro. It is the second busiest metro system in Europe after Moscow Metro, notably, Paris Gare du Nord is the busiest railway station in the world outside of Japan, with 262 millions passengers in 2015. In 2015, Paris received 22.2 million visitors, making it one of the top tourist destinations. The association football club Paris Saint-Germain and the rugby union club Stade Français are based in Paris, the 80, 000-seat Stade de France, built for the 1998 FIFA World Cup, is located just north of Paris in the neighbouring commune of Saint-Denis. Paris hosts the annual French Open Grand Slam tennis tournament on the red clay of Roland Garros, Paris hosted the 1900 and 1924 Summer Olympics and is bidding to host the 2024 Summer Olympics. The name Paris is derived from its inhabitants, the Celtic Parisii tribe. Thus, though written the same, the name is not related to the Paris of Greek mythology. In the 1860s, the boulevards and streets of Paris were illuminated by 56,000 gas lamps, since the late 19th century, Paris has also been known as Panam in French slang. Inhabitants are known in English as Parisians and in French as Parisiens and they are also pejoratively called Parigots. The Parisii, a sub-tribe of the Celtic Senones, inhabited the Paris area from around the middle of the 3rd century BC. One of the areas major north-south trade routes crossed the Seine on the île de la Cité, this place of land and water trade routes gradually became a town
11.
France
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France, officially the French Republic, is a country with territory in western Europe and several overseas regions and territories. The European, or metropolitan, area of France extends from the Mediterranean Sea to the English Channel and the North Sea, Overseas France include French Guiana on the South American continent and several island territories in the Atlantic, Pacific and Indian oceans. France spans 643,801 square kilometres and had a population of almost 67 million people as of January 2017. It is a unitary republic with the capital in Paris. Other major urban centres include Marseille, Lyon, Lille, Nice, Toulouse, during the Iron Age, what is now metropolitan France was inhabited by the Gauls, a Celtic people. The area was annexed in 51 BC by Rome, which held Gaul until 486, France emerged as a major European power in the Late Middle Ages, with its victory in the Hundred Years War strengthening state-building and political centralisation. During the Renaissance, French culture flourished and a colonial empire was established. The 16th century was dominated by civil wars between Catholics and Protestants. France became Europes dominant cultural, political, and military power under Louis XIV, in the 19th century Napoleon took power and established the First French Empire, whose subsequent Napoleonic Wars shaped the course of continental Europe. Following the collapse of the Empire, France endured a succession of governments culminating with the establishment of the French Third Republic in 1870. Following liberation in 1944, a Fourth Republic was established and later dissolved in the course of the Algerian War, the Fifth Republic, led by Charles de Gaulle, was formed in 1958 and remains to this day. Algeria and nearly all the colonies became independent in the 1960s with minimal controversy and typically retained close economic. France has long been a centre of art, science. It hosts Europes fourth-largest number of cultural UNESCO World Heritage Sites and receives around 83 million foreign tourists annually, France is a developed country with the worlds sixth-largest economy by nominal GDP and ninth-largest by purchasing power parity. In terms of household wealth, it ranks fourth in the world. France performs well in international rankings of education, health care, life expectancy, France remains a great power in the world, being one of the five permanent members of the United Nations Security Council with the power to veto and an official nuclear-weapon state. It is a member state of the European Union and the Eurozone. It is also a member of the Group of 7, North Atlantic Treaty Organization, Organisation for Economic Co-operation and Development, the World Trade Organization, originally applied to the whole Frankish Empire, the name France comes from the Latin Francia, or country of the Franks
