Middleton High School is a public secondary school located in the city of Middleton, Wisconsin. It was established in 1879. Part of the Middleton-Cross Plains School District, the school serves more than 2,000 students in grades 9 to 12 from the Middleton and Cross Plains areas. Middleton High School's mascot is the cardinal. The school colors are white and cardinal red. Its athletic teams play at the WIAA Division 1 level in the WIAA Big Eight Conference. Middleton High School is often praised for having highly rated STEM and athletics programs.

Middleton is a city in Dane County, Wisconsin, United States, and a suburb of the state capital, Madison. Middleton's motto is "The Good Neighbor City." The population was 21,827 at the 2020 census.