Middletown is a town in Frederick County, Virginia, United States, in the northern Shenandoah Valley. The population was 1,265 at the 2010 census, up from 1,015 at the 2000 census.

Frederick County is located in the Commonwealth of Virginia. As of the 2020 census, the population was 91,419. Its county seat is Winchester. The county was formed in 1743 by the splitting of Orange County. It is Virginia's northernmost county. Frederick County is included in the Winchester, VA-WV Metropolitan Statistical Area, which is also included in the Washington-Baltimore-Northern Virginia, DC-MD-VA-WV-PA Combined Statistical Area.