Route 13 is a highway in Missouri which runs almost the entire north–south length of the state. Its northern terminus is at U.S. Route 69/136 in Bethany. Its southern terminus is at the Arkansas state line in downtown Blue Eye, Missouri–Arkansas where it continues as Highway 21. It is one of the original state highways of Missouri.

Greene County is located in the southwest part of the U.S. state of Missouri. As of the 2020 census, its population was 298,915. making it the fourth most-populous county in Missouri.