Moccasin Gap, also known as Big Moccasin Gap, is a pass in Clinch Mountain, a long ridge within the Appalachian Mountains, at Gate City, Virginia. This gap has a long history as a passageway through the mountain. It was used by the Cherokee and Shawnee, and was the first gap through which the Daniel Boone Wilderness Road passed on its way to the better-known Cumberland Gap and Kentucky. Today it serves as a primary commercial route for industry, retail, and tourism businesses.

Clinch Mountain is a mountain ridge in the U.S. states of Tennessee and Virginia, lying in the ridge-and-valley section of the Appalachian Mountains. From its southern terminus at Kitts Point, which lies at the intersection of Knox, Union and Grainger counties near Blaine, Tennessee, it runs in a generally east-northeasterly direction to Garden Mountain near Burke's Garden, Virginia. It separates the Clinch River basin to the north and the Holston River basin to the south.