Moosonee is a town in northern Ontario, Canada, on the Moose River approximately 19 km (12 mi) south of James Bay. It is considered to be "the Gateway to the Arctic" and has Ontario's only saltwater port. Nearby on Moose Factory Island is the community of Moose Factory to which it is connected by water taxi in the summer and ice road in the winter.

The Moose River is a river in the Hudson Plains ecozone of northern Ontario, Canada. The river flows 100 km northeast from the confluence of the Mattagami and Missinaibi Rivers into James Bay. Its drainage basin is 108,500 square kilometres (41,900 sq mi) and it has a mean discharge rate of 1,370 cubic metres (48,000 cu ft). Its full length is 547 kilometres (340 mi) if counted from the head of the Mattagami River.