Mosley Street was a tram stop in the City Zone of Greater Manchester's Metrolink light rail system which closed on 18 May 2013. It was located on Mosley Street in Manchester city centre and was the last unidirectional stop on the Metrolink, with a single platform serving southbound passengers travelling towards Altrincham Interchange, Eccles Interchange, MediaCityUK and St Werburgh's Road only.

Zone 1 of the Manchester Metrolink light rail network is the heart of the system where all of the other lines converge. Its boundaries are broadly equivalent to those of Manchester city centre, and approximately mirror the city's Inner Ring Road. Within Zone 1, first opened in 1992 as the City Zone, trams largely run along semi-pedestrianised streets rather than on their own separate alignment. The original route between the Altrincham and Bury lines ran to Victoria station via Market Street and High Street, and was soon joined by a branch to Piccadilly station by a three-way delta junction. A second route between the South-West and North-Eastern parts of the network was built to ease congestion on the original line. Opened in 2017, the Second City Crossing (2CC) added one additional stop to the network.