Moston Brook is a stream in Greater Manchester in north-west England and a tributary of the River Irk. The brook is formed at the confluence of Bower Brook and Hole Bottom Brook. This occurs near the Rochdale Canal in Failsworth in the Metropolitan Borough of Oldham. It flows southwest, forming the border between Moston, Manchester and Failsworth before being culverted almost all of the remaining route to its meeting the River Irk. It has a total length of about 3.7 miles.

The River Irk is a river in the historic county of Lancashire in the North West England that flows through the northern most Lancastrian towns of the ceremonial county of Greater Manchester.