Mount Lemmon Observatory (MLO), also known as the Mount Lemmon Infrared Observatory, is an astronomical observatory located on Mount Lemmon in the Santa Catalina Mountains approximately 28 kilometers (17 mi) northeast of Tucson, Arizona (US). The site in the Coronado National Forest is used with special permission from the U.S. Forest Service by the University of Arizona's Steward Observatory, and contains a number of independently managed telescopes.

Steward Observatory is the research arm of the Department of Astronomy at the University of Arizona (UArizona). Its offices are located on the UArizona campus in Tucson, Arizona (US). Established in 1916, the first telescope and building were formally dedicated on April 23, 1923. It now operates, or is a partner in telescopes at five mountain-top locations in Arizona, one in New Mexico, one in Hawaii, and one in Chile. It has provided instruments for three different space telescopes and numerous terrestrial ones. Steward also has one of the few facilities in the world that can cast and figure the very large primary mirrors used in telescopes built in the early 21st century.