The Musquodoboit Valley is a valley and region in the Halifax Regional Municipality of Nova Scotia, Canada. It is administratively located in the municipality's Musquodoboit Valley & Dutch Settlement planning area and the western edge of the valley includes communities that are considered part of the commutershed for the urban area of the Halifax Regional Municipality. The picturesque Musquodoboit River flows through majority of the valley, passing by most of the communities in the valley. The river is approximately 97 kilometres (60 mi) long and originates in the extreme northeastern area of the valley. The three largest communities in the valley are Upper Musquodoboit, Middle Musquodoboit and Musquodoboit Harbour. The word "Musquodoboit" is derived from the Mi’kmaq language and means "rolling out in foam".

Halifax is the capital and most populous municipality of the Canadian province of Nova Scotia, and the most populous municipality in Atlantic Canada. As of 2022, it is estimated that the population of the Halifax CMA was 480,582, with 348,634 people in its urban area. The regional municipality consists of four former municipalities that were amalgamated in 1996: Halifax, Dartmouth, Bedford, and Halifax County.