Napanee station in Napanee, Ontario, Canada is served by Via Rail trains running from Toronto to Ottawa. The 1856 limestone railway station was an unstaffed but heated shelter with telephones and washrooms, which would open at least half an hour before a train arrives. The platform is wheelchair-accessible. As of February 2023, the shelter was locked.

Canadian National Railway's Kingston Subdivision, or Kingston Sub for short, is a major railway line connecting Toronto with Montreal that carries the majority of CN traffic between these points. The line was originally the main trunk for the Grand Trunk Railway between these cities, although there has been some realignment of the route between these cities. The majority of the Kingston Sub runs close to the northern bank of Lake Ontario and the Saint Lawrence River.