Neithhotep or Neith-hotep was an ancient Egyptian queen consort who lived and ruled during the early First Dynasty. She was once thought to be a male ruler: her outstandingly large mastaba and the royal serekh surrounding her name on several seal impressions previously led Egyptologists and historians to the erroneous belief that she might have been an unknown king. As the understanding of early Egyptian writings developed, scholars learned that Neithhotep was in fact a woman of extraordinary rank. She was subsequently considered to be the wife of unified Egypt's first pharaoh, Narmer, and the mother of Hor-Aha.

Djer is considered the third pharaoh of the First Dynasty of ancient Egypt in current Egyptology. He lived around the mid 31st century BC and reigned for c. 40 years. A mummified forearm of Djer or his wife was discovered by Egyptologist Flinders Petrie, but was discarded by Émile Brugsch.