New York State Railways was a subsidiary of the New York Central Railroad that controlled several large city streetcar and electric interurban systems in upstate New York. It included the city transit lines in Rochester, Syracuse, Utica, Oneida and Rome, plus various interurban lines connecting those cities. New York State Railways also held a 50% interest in the Schenectady Railway Company, but it remained a separate independent operation. The New York Central took control of the Rochester Railway Company, the Rochester and Eastern Rapid Railway and the Rochester and Sodus Bay Railway in 1905, and the Mohawk Valley Company was formed by the railroad to manage these new acquisitions. New York State Railways was formed in 1909 when the properties controlled by the Mohawk Valley Company were merged. In 1912 it added the Rochester and Suburban Railway, the Syracuse Rapid Transit Railway, the Oneida Railway, and the Utica and Mohawk Valley Railway. The New York Central Railroad was interested in acquiring these lines in an effort to control the competition and to gain control of the lucrative electric utility companies that were behind many of these streetcar and interurban railways. Ridership across the system dropped through the 1920s as operating costs continued to rise, coupled with competition from better highways and private automobile use. New York Central sold New York State Railways in 1928 to a consortium led by investor E. L. Phillips, who was looking to gain control of the upstate utilities. Phillips sold his stake to Associated Gas & Electric in 1929, and the new owners allowed the railway bonds to default. New York State Railways entered receivership on December 30, 1929. The company emerged from receivership in 1934, and local operations were sold off to new private operators between 1938 and 1948.

The Rochester Railway Company operated a streetcar transit system throughout the city of Rochester from 1890 until its acquisition by Rochester Transit Corp. in 1938. Formed by a group of Pittsburgh investors, the Rochester Railway Company purchased the Rochester City & Brighton Railroad in 1890, followed by a lease of the Rochester Electric Railway in 1894. The Rochester and Suburban Railway was leased in 1905, extending the system's reach to Irondequoit and Sea Breeze. Rochester Railways was acquired by the Mohawk Valley Company, a subsidiary of the New York Central Railroad set up to take control of electric railways in its territory. In 1909 the holdings of the Mohawk Valley Company were consolidated as the New York State Railways.