New York State Route 327 (NY 327) is a state highway located within Tompkins County, New York, in the United States. It extends for 7.11 miles (11.44 km) in a northwest–southeast direction from an intersection with the concurrent routes of NY 13, NY 34, and NY 96 in the town of Ithaca to a junction with NY 79 in the town of Enfield. NY 327 runs along the northern edge of Robert H. Treman State Park as it passes from Ithaca to Enfield. The route was assigned as part of the 1930 renumbering of state highways in New York.

Tompkins County is a county located in the U.S. state of New York. As of the 2020 census, the population was 105,740. The county seat is Ithaca. The name is in honor of Daniel D. Tompkins, who served as Governor of New York and Vice President of the United States. The county is part of the Southern Tier region of the state.