New York State Route 329 (NY 329) is a 2.57-mile-long (4.14 km) state highway near Watkins Glen, New York, in the United States. The western terminus is at an intersection with Meads Hill Road in Dix. The eastern terminus is at a junction with NY 14 and NY 414 in Watkins Glen. NY 329 passes south of Watkins Glen State Park while New York State Route 419, a highway 0.30 miles (0.48 km) in length, connects NY 329 to the park. Both NY 329 and NY 419 pass through sparsely populated, rural areas.

Watkins Glen is a village and census-designated place in and the county seat of Schuyler County, New York, United States. As of the 2020 census, the population was 1,829. Watkins Glen lies within the towns of Dix and Reading. To the southwest of the village is the Watkins Glen International race track, which hosts annual NASCAR Cup Series and WeatherTech SportsCar Championship races, and formerly hosted the Formula One United States Grand Prix and various IndyCar races.