New York State Route 65 (NY 65) is a north–south state highway located in the western portion of New York in the United States. It extends for 18.51 miles (29.79 km) from an intersection with U.S. Route 20 (US 20) and NY 5 in the Ontario County town of West Bloomfield to a junction with NY 96 in the Monroe County town of Brighton. In between, the route serves the village of Honeoye Falls and passes through the extreme northeastern corner of Livingston County. NY 65 intersects NY 251 in Mendon, NY 252 in Pittsford, and the regionally important NY 31 in Brighton. The southern half of NY 65 passes through mostly rural areas, while its northern section traverses densely populated portions of Monroe County.

U.S. Route 20 (US 20) is a part of the United States Numbered Highway System that runs from Newport, Oregon, to Boston, Massachusetts. In the U.S. state of New York, US 20 extends 372.32 miles (599.19 km) from the Pennsylvania state line at Ripley to the Massachusetts state line in the Berkshires. US 20 is the longest surface road in New York. It runs near the Lake Erie shore from Ripley to Buffalo and passes through the southern suburbs of Buffalo, the Finger Lakes, the glacial moraines of Central New York, and the city of Albany before crossing into Massachusetts. US 20 connects to all three major north–south Interstate Highways in Upstate New York: Interstate 390 (I-390) near Avon, I-81 south of Syracuse, and I-87 in Albany by way of NY 910F.