The Nilgiri Biosphere Reserve is a biosphere reserve in the Nilgiri Mountains of the Western Ghats in South India. It is the largest protected forest area in India, spreading across Tamil Nadu, Karnataka and Kerala. It includes the protected areas Mudumalai National Park, Mukurthi National Park, Sathyamangalam Wildlife Sanctuary in Tamil Nadu; Nagarhole National Park, Bandipur National Park, both in Karnataka; Silent Valley National Park, Aralam Wildlife Sanctuary, Wayanad Wildlife Sanctuary, and Karimpuzha Wildlife Sanctuary in Kerala.

The Nilgiri Mountains form part of the Western Ghats in northwestern Tamil Nadu, Southern Karnataka, and eastern Kerala in India. They are located at the trijunction of three states and connect the Western Ghats with the Eastern Ghats. At least 24 of the Nilgiri Mountains' peaks are above 2,000 metres (6,600 ft), the highest peak being Doddabetta, at 2,637 metres (8,652 ft).