Northwestern Ontario is a secondary region of Northern Ontario in the Canadian province of Ontario which lies north and west of Lake Superior and west of Hudson Bay and James Bay. It includes most of subarctic Ontario. Its western boundary is the Canadian province of Manitoba, which disputed Ontario's claim to the western part of the region. Ontario's right to Northwestern Ontario was determined by the Judicial Committee of the Privy Council in 1884 and confirmed by the Canada Act, 1889, of the Parliament of the United Kingdom. In 1912, the Parliament of Canada by the Ontario Boundaries Extension Act gave jurisdiction over the District of Patricia to Ontario, thereby extending the northern boundary of the province to Hudson Bay.

Northern Ontario is a primary geographic and quasi-administrative region of the Canadian province of Ontario, the other primary region being Southern Ontario. Most of the core geographic region is located on part of the Superior Geological Province of the Canadian Shield, a vast rocky plateau located mainly north of Lake Huron, the French River, Lake Nipissing, and the Mattawa River. The statistical region extends south of the Mattawa River to include all of the District of Nipissing. The southern section of this district lies on part of the Grenville Geological Province of the Shield which occupies the transitional area between Northern and Southern Ontario. The extended federal and provincial quasi-administrative regions of Northern Ontario have their own boundaries even further south in the transitional area that vary according to their respective government policies and requirements. Ontario government departments and agencies such as the Growth Plan for Northern Ontario and the Northern Ontario Heritage Fund Corporation define Northern Ontario as all areas north of, and including, the districts of Parry Sound and Nipissing for political purposes, and the federal but not the provincial government also includes the district of Muskoka.