Nuclear magnetic resonance
Videos
Nuclear magnetic resonance is a physical phenomenon in which nuclei in a strong constant magnetic field are disturbed by a weak oscillating magnetic field and respond by producing an electromagnetic s
Bruker 700 MHz nuclear magnetic resonance (NMR) spectrometer.
900 MHz, 21.2 T NMR Magnet at HWB-NMR, Birmingham, UK
Medical MRI
Nuclear magnetic resonance spectroscopy of proteins is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucl
The NMR sample is prepared in a thin-walled glass tube.
Comparison of a COSY and TOCSY 2D spectra for an amino acid like glutamate or methionine. The TOCSY shows off diagonal crosspeaks between all protons in the spectrum, but the COSY only has crosspeaks between neighbours.
Schematic of an HNCA and HNCOCA for four sequential residues. The nitrogen-15 dimension is perpendicular to the screen. Each window is focused on the nitrogen chemical shift of that amino acid. The sequential assignment is made by matching the alpha carbon chemical shifts. In the HNCA each residue sees the alpha carbon of itself and the preceding residue. The HNCOCA only sees the alpha carbon of the preceding residue.
Nuclear magnetic resonance structure determination generates an ensemble of structures. The structures will converge only if the data is sufficient to dictate a specific fold. In these structures, it is the case for only a part of the structure. From PDB entry 1SSU.