State Route 29 is an east–west state highway in the west-central portion of the U.S. state of Ohio. Its western terminus is at the Indiana state line near Celina, where State Road 67 continues west. It continues east to St. Marys where it junctions with U.S. Route 33. In that town, it also crosses State Route 66, State Route 116, and State Route 703, which was its former alignment before a divided highway was built. After turning south it crosses State Route 219 in New Knoxville and then has an interchange with Interstate 75, continuing into Sidney where it meets State Route 47. Still going southeast, it briefly joins State Route 235 before turning east and then south again to enter Urbana. Here the route joins U.S. Route 36, and the concurrency intersects with U.S. Route 68 and State Route 54. From there, State Route 29 leaves U.S. Route 36 and continues to Mutual, intersecting with State Route 161, and State Route 56 shortly after; later, in Mechanicsburg, the route intersects with State Route 4. The route then intersects with State Route 38, U.S. Route 42, and Interstate 70 before reaching its eastern terminus at U.S. Route 40 on the western edge of West Jefferson.

U.S. Route 33 (US 33) is a United States Numbered Highway running from near Elkhart, Indiana, to Richmond, Virginia. Within the state of Ohio, it is a predominantly southeast–northwest highway running from west of Willshire before crossing over into West Virginia via the Ravenswood Bridge over the Ohio River. The route runs through largely rural territory throughout most of the state's west-central, central, and southeastern regions, although it also passes through large portions of downtown Columbus.