State Route 41 is a north–south state highway in the southern and western portions of the U.S. state of Ohio. Its southern terminus is at U.S. Route 52, US 62 Business, and US 68 Business in Aberdeen. ; and its northern terminus is along SR 48 at US 36 in Covington. Throughout its southern portion the route is quite hilly as it passes predominantly northward through scenic areas with state parks and monuments. Along its northern portion the route heads in a more westerly direction across mainly flat terrain as a major road through the cities of Washington Court House, Springfield, and Troy.

U.S. Route 52 runs east–west across the southern part of the state of Ohio along the Ohio River, passing through or very near the cities and towns of Cincinnati, Portsmouth, and Ironton. For its first 19 miles (31 km) or so, the highway runs concurrently with Interstate 74 (I-74) and I-75 before it winds through downtown Cincinnati for several miles. The route is primarily two lanes between New Richmond and West Portsmouth, Ohio, where it becomes a four-lane partial access highway until it exits the state near Chesapeake.