The "Old Three Hundred" were 297 grantees who purchased 307 parcels of land from Stephen Fuller Austin in Mexican Texas. Each grantee was head of a household, or, in some cases, a partnership of unmarried men. Austin was an American approved in 1822 by Mexico as an empresario for this effort, after the nation had gained independence from Spain. By 1825 the colony had a population of 1,790, including 443 enslaved African Americans. Because the Americans believed they needed enslaved workers, Austin negotiated with the Mexican government to gain approval, as the new nation was opposed to slavery. Mexico abolished it in 1837.

Stephen Fuller Austin was an American-born empresario. Known as the "Father of Texas" and the founder of Anglo Texas, he led the second and, ultimately, the successful colonization of the region by bringing 300 families and their slaves from the United States to the Tejas region of Mexico in 1825.