Once Upon a Studio is a 2023 American live-action/animated crossover fantasy comedy short film produced by Walt Disney Animation Studios in celebration of the Walt Disney Company's centennial. Written and directed by Dan Abraham and Trent Correy, it was released by Walt Disney Pictures on October 16, 2023. In the film, Disney characters come to life from pictures hanging on the walls of the Roy E. Disney Animation Building following the end of a usual work day. The short's art style combines computer graphics, traditional animation and live-action, and features characters from the majority of the studio's works made up to that point, including all 62 feature films at the time of the short's release, numerous short films, and some live-action Disney films featuring animation produced by the studio such as The Reluctant Dragon (1941), Mary Poppins (1964), Bedknobs and Broomsticks (1971), and Pete's Dragon (1977). The film was dedicated in memory of Burny Mattinson, the company's longest-serving employee, who made a cameo in the short and died eight months before its release.

Tinker Bell is a fictional fairy based on the same name character created in 1904 by Scottish novelist and playwright J. M. Barrie, and one of the most popular characters adapted for film and television by The Walt Disney Company. She first appeared in the 1953 Disney animated film, Peter Pan, and has since become a widely recognized unofficial mascot of Disney, next to the company's official mascot Mickey Mouse. She is recognized as "a symbol of 'the magic of Disney'".