The 2007 One Night Stand was the third annual One Night Stand professional wrestling pay-per-view (PPV) event produced by World Wrestling Entertainment (WWE). It was held for wrestlers from the promotion's Raw, SmackDown!, and ECW brand divisions. The event took place on June 3, 2007, at the Jacksonville Veterans Memorial Arena in Jacksonville, Florida. While the previous two years' events were held primarily as reunion shows for Extreme Championship Wrestling (ECW) alumni, the 2007 event was promoted as a WWE event with wrestlers from all three brands participating as following WrestleMania 23, brand-exclusive PPVs were discontinued. It was the final event to announce each individual brand as co-promoters of the PPV during the introductory graphics.

VyStar Veterans Memorial Arena is a multi-purpose arena located in Jacksonville, Florida. It currently serves as the home arena of the Jacksonville Icemen of the ECHL, the Jacksonville Giants of the American Basketball Association, and the Jacksonville Sharks of the National Arena League.