Most organisms involved in water purification originate from the waste, wastewater or water stream itself or arrive as resting spore of some form from the atmosphere. In a very few cases, mostly associated with constructed wetlands, specific organisms are planted to maximise the efficiency of the process.

Waste stabilization ponds are ponds designed and built for wastewater treatment to reduce the organic content and remove pathogens from wastewater. They are man-made depressions confined by earthen structures. Wastewater or "influent" enters on one side of the waste stabilization pond and exits on the other side as "effluent", after spending several days in the pond, during which treatment processes take place.