Oswaldtwistle Rovers Football Club were a team based in the town of Oswaldtwistle, Lancashire. They first entered the FA Cup in 1884 and, in 1885, reached the second round. Rovers were one of the founder members of the Lancashire League in 1889, but predominantly competed in the Lancashire Combination between 1894 and 1909. In 1909, after leaving the second division of the Combination, they played their final game in the FA Cup.

Oswaldtwistle is a town in the Hyndburn borough of Lancashire, England, 3 miles (5 km) southeast of Blackburn, contiguous with Accrington and Church. The town has a rich industrial heritage, being home to James Hargreaves, inventor of the spinning jenny and Sir Robert Peel of calico printing fame. The Leeds and Liverpool Canal passes through the town and was responsible for the export of much of the area's cotton produce.