Otago Harbour is the natural harbour of Dunedin, New Zealand, consisting of a long, much-indented stretch of generally navigable water separating the Otago Peninsula from the mainland. They join at its southwest end, 21 km (13 mi) from the harbour mouth. It is home to Dunedin's two port facilities, Port Chalmers and at Dunedin's wharf. The harbour has been of significant economic importance for approximately 700 years, as a sheltered harbour and fishery, then deep water port.

Dunedin is the second-largest city in the South Island of New Zealand, and the principal city of the Otago region. Its name comes from Dùn Èideann, the Scottish Gaelic name for Edinburgh, the capital of Scotland. The city has a rich Māori, Scottish, and Chinese heritage.