Owensboro is a home rule-class city in and the county seat of Daviess County, Kentucky, United States. It is the fourth-most populous city in the state. Owensboro is located on U.S. Route 60 and Interstate 165 about 107 miles (172 km) southwest of Louisville, and is the principal city of the Owensboro metropolitan area. The 2020 census had its population at 60,183. The metropolitan population was estimated at 116,506. The metropolitan area is the sixth largest in the state as of 2018, and the seventh largest population center in the state when including micropolitan areas.

Daviess County ( "Davis"), is a county in Kentucky. As of the 2020 census, the population was 103,312. Its county seat is Owensboro. The county was formed from part of Ohio County on January 14, 1815.