Palatinate-Neuburg was a territory of the Holy Roman Empire, founded in 1505 by a branch of the House of Wittelsbach. Its capital was Neuburg an der Donau. Its area was about 2,750 km2, with a population of some 100,000.

Count Palatine Wolfgang of Zweibrücken was member of the Wittelsbach family of the Counts Palatine and Duke of Zweibrücken from 1532. With the support of his regent, his uncle Rupert, Wolfgang introduced the Reformation to Zweibrücken in 1537.