Pauline Rita, born Margaret Glenister, was an English soprano and actress. During her early career, she was best known for her performances in operettas and comic operas at the Opera Comique and was associated with impresario Richard D'Oyly Carte. Later she married flautist John Radcliff, and the two performed together for many years.

The Opera Comique was a 19th-century theatre constructed in Westminster, London, located between Wych Street, Holywell Street and the Strand. It opened in 1870 and was demolished in 1902, to make way for the construction of the Aldwych and Kingsway.