Pennsylvania Route 249 is a 22.1-mile-long (35.6 km) state highway located in Tioga County, Pennsylvania. The southern terminus is at PA 287 in Middlebury Township. The northern terminus is at New York State Route 36 at the New York state line north of Knoxville.

Tioga County is a county in the Commonwealth of Pennsylvania. As of the 2020 census, the population was 41,045. Its county seat is Wellsboro. The county was created on March 26, 1804, from part of Lycoming County and later organized in 1812. It is named for the Tioga River. The county is part of the North Central Pennsylvania region of the state.