Pennsylvania Route 325 is a 30-mile-long (48 km) state highway located in Dauphin and Schuylkill counties in Pennsylvania. The western terminus is at the intersection of Mountain Road and Earl Drive, just past an interchange with U.S. Route 22 /US 322, in the community of Speeceville in Middle Paxton Township. The eastern terminus is at US 209 in Tower City. PA 325 runs through a narrow valley for its length, passing through forested areas with some farmland. The route forms a concurrency with PA 225 to the north of Dauphin. The road was paved in stages between 1930 and the 1940s. PA 325 was designated in the 1940s to run between US 22/US 322/PA 14 in Speeceville east to US 209 in Tower City. In 1999, an interchange with US 22/US 322 was constructed near the western terminus; the end of PA 325 remained at Riverview Road, the former alignment of US 22/US 322.

Dauphin County is a county in the Commonwealth of Pennsylvania. As of the 2020 census, the population was 286,401. The county seat is Harrisburg, Pennsylvania's state capital and ninth-most populous city. The county was created on March 4, 1785, from part of Lancaster County and was named after Louis Joseph, Dauphin of France, the first son of King Louis XVI. The county is part of the South Central Pennsylvania region of the state.