Pennsylvania Route 328 is a 13.2-mile-long (21.2 km) state highway located in Tioga and Bradford counties in Pennsylvania. The western terminus is at PA 287 in Tioga Junction. The eastern terminus is at New York State Route 328 (NY 328) at the New York state line near Millerton.

Tioga County is a county in the Commonwealth of Pennsylvania. As of the 2020 census, the population was 41,045. Its county seat is Wellsboro. The county was created on March 26, 1804, from part of Lycoming County and later organized in 1812. It is named for the Tioga River. The county is part of the North Central Pennsylvania region of the state.