Pennsylvania Route 427 is a 10.7-mile-long (17.2 km) state highway located in Venango and Crawford counties in Pennsylvania. The southern terminus is at U.S. Route 322 in Sugarcreek. The northern terminus is at PA 27 near Bradleytown.

Venango County is a county in the Commonwealth of Pennsylvania. As of the 2020 census, the population was 50,454. Its county seat is Franklin. The county was created in 1800 and later organized in 1805. The county is part of the Northwest Pennsylvania region of the state.