Pennsylvania Route 616 is a 14.9-mile-long (24.0 km) state highway located in York County in Pennsylvania. The southern terminus is at PA 851 in Railroad. The northern terminus is at U.S. Route 30 in West Manchester Township. PA 616 is a two-lane undivided road that passes through rural areas in southwestern York County, serving the communities of Glen Rock, Seven Valleys, and New Salem. The route forms a concurrency with PA 216 in Glen Rock and intersects the western terminus of PA 214 in Seven Valleys.

York County is a county in the Commonwealth of Pennsylvania, United States. As of the 2020 census, the population was 456,438. Its county seat is York. The county was created on August 19, 1749, from part of Lancaster County and named either after the Duke of York, an early patron of the Penn family, or for the city and county of York in England. The county is part of the South Central region of the state.