Pennsylvania Route 65 is a major 51-mile-long (82 km) state highway located in western Pennsylvania, United States. The route, traveling north–south from the Interstate 279/U.S. Route 19 Truck concurrency in Pittsburgh north to the PA 108/PA 168 concurrency in New Castle, connects downtown Pittsburgh to the northwestern portion of the Pittsburgh Metropolitan Area. PA 65 is similar in its purpose to PA 18 and PA 51, both of which run parallel to PA 65 at one point or another; however, the three routes pass through different cities for most of their respective alignments.

Interstate 279 (I-279), locally referred to as Parkway North, is a north–south auxiliary Interstate Highway that lies entirely within Allegheny County, Pennsylvania. Its southern end is at I-376 at the Fort Pitt Bridge in Pittsburgh, and the north end is in Franklin Park at I-79. It primarily serves at the main access route between Pittsburgh and its northern suburbs.