Peno is an urban-type settlement and the administrative center of Penovsky District of Tver Oblast, Russia. It is located between Lake Peno and Lake Volgo, both located in the course of the Volga River and belonging to Upper Volga Reservoir. Population: 4,220 (2010 Russian census); 5,358 (2002 Census); 6,212 (1989 Soviet census).

Penovsky District is an administrative and municipal district (raion), one of the thirty-six in Tver Oblast, Russia. It is located in the northwest of the oblast and borders with Ostashkovsky District in the north, Selizharovsky District in the east, Andreapolsky District in the south, and with Maryovsky District of Novgorod Oblast in the west. The area of the district is 2,385 square kilometers (921 sq mi). Its administrative center is the urban locality of Peno. Population: 6,864 ; 8,523 (2002 Census); 10,291 (1989 Soviet census). The population of Peno accounts for 61.5% of the district's total population.